Real-time fluorescence quantitative PCR (quantitative PCR, qPCR) realizes the qualitative and quantitative analysis of the initial template through the real-time monitoring of the fluorescence signal of each cycle product in the PCR amplification reaction. At present, PCR products are mainly labeled and tracked through fluorescent dyes or fluorescent-labeled probes, the reaction process is in-situ real-time monitored, and the products are analyzed in combination with the corresponding software to calculate the initial concentration of the template of the sample to be tested. The real-time fluorescence quantitative PCR detection method has a wide range of applications in biology and medicine.

Spectral imaging technology can provide fast, real-time, efficient, and non-destructive measurements, and can be integrated into PCR, fluorescence, Raman or other devices for rapid detection, improving the current detection level and efficiency.