实时荧光定量PCR（quantitative PCR, qPCR）通过对PCR扩增反应中的每一个循环产物荧光信号的实时监测，实现对起始模板的定性及定量分析。目前主要通过荧光染料或者荧光标记的探针对PCR产物进行标记跟踪，实时在线监控反应过程，并结合相应的软件对产物进行分析，计算待测样品模板的初始浓度。实时荧光定量PCR检测法在生物学、医药学上都有着广泛的应用。

光谱成像技术可以提供快速、实时、高效、非破坏性测量，同时可集成到PCR、荧光、拉曼或其他设备中，进行快速检测，提升当前检测水平及效率。

Real-time fluorescence quantitative PCR (quantitative PCR, qPCR) realizes the qualitative and quantitative analysis of the initial template through the real-time monitoring of the fluorescence signal of each cycle product in the PCR amplification reaction. At present, PCR products are mainly labeled and tracked through fluorescent dyes or fluorescent-labeled probes, the reaction process is in-situ real-time monitored, and the products are analyzed in combination with the corresponding software to calculate the initial concentration of the template of the sample to be tested. The real-time fluorescence quantitative PCR detection method has a wide range of applications in biology and medicine.

Spectral imaging technology can provide fast, real-time, efficient, and non-destructive measurements, and can be integrated into PCR, fluorescence, Raman or other devices for rapid detection, improving the current detection level and efficiency.